Technical note: viability and motility of vitrified/thawed primordial germ cell isolated from common carp (Cyprinus carpio) somite embryos.

Running head: Motility of vitrified primordial germ cell Technical note: Viability and motility of vitrified/thawed primordial germ cell isolated from common carp (Cyprinus carpio) somite embryos

Molecular cloning and developmental expression patterns of the MyoD and MEF2 families of muscle transcription factors in the carp.

cDNA clones encoding the myogenic regulatory factors (MRFs) myogenin, MyoD and myf-5 were isolated by reverse-transcription polymerase chain reaction from larvae and embryos of the common carp (Cyprinus carpio L.). Myocyte-specific enhancer factor 2 (MEF2) cDNAs were identified from a cDNA library from adult carp. Northern blot analysis showed that MyoD, myf-5 and MEF2C transcripts were present...

Cryopreservation of common carp (Cyprinus carpio) embryos using different cryoprotectant

This study reports that common carp (Cyprinus carpio) embryos were successfully frozen and preserved by vitrification. The modified Haga solution was tested by using two different cryoprotectants and three different concentrations at 4°C to obtain appropriate vitrified solutions ((dimethylsulfoxide 5, 10, 15% (DMSO) and glycerol 5, 10, 15% (gly)) in the study.  The hatching rates of embryos exp...

The differentiation of germ cells and gonads during development of carp (Cyprinus carpio L.). A study with anti-carp sperm monoclonal antibodies.

Gonadal development, germ cell differentiation and the appearance of membrane antigenic determinants, specific for male and female germ cells during gonadogenesis, was studied in larval and juvenile carp (Cyprinus carpio L.) until 25 weeks after fertilization. Indirect immunofluorescence studies with four monoclonal antibodies raised against carp spermatozoa revealed that monoclonal antibody WC...

Viability of Male Gametes in Common Carp (Cyprinus carpio) along the Lower Colorado River from the Cibola National Wildlife Refuge (NWR), Havasu NWR, and Lake Mohave of Lake Mead National Recreation Area

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